CD3 epsilon Antibody

• Catalog Number: orb248225
• Category: Antibodies
• Description: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody. This sequence is from the cytoplasmic portion of the protein.
• Tested applications: WB
• Dilution range: Western blot: 1-2ug/ml for 60 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.

Western blot testing of Jurkat lysate with CD3e antibody.