CD3 antibody

• Catalog Number: orb421969
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3
• Clonality: Monoclonal
• Clone Number: TB3
• Tested applications: FC, ICC
• Reactivity: Human
• Isotype: Mouse IgG2b
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD3E antibody, anti-T3E antibody, anti-TCRE a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (TB3) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (TB3) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.

Immunocytochemistry staining of human CD3 epsilon and CD3 gamma transfected COS cells using mouse monoclonal antibodies anti-CD3, clones UCHT1 (left) and TB3 (right) indicated by Alexa Fluor 555 signal (red); DNA stained by DAPI (blue).