CD3 antibody (FITC)

• Catalog Number: orb545695
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: OKT3
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: human T cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD3E antibody, anti-T3E antibody, anti-TCRE a
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (OKT3) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (OKT3) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).