CD3 activation epitope antibody (FITC)

• Catalog Number: orb507496
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD3 activation epitope conjugated to FITC
• Clonality: Monoclonal
• Clone Number: APA1/1
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified human CD3 proteins isolated from thymus
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD3 epsilon (activation epitope)
• Entrez: 916
• UniProt ID: P07766
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD3 epsilon chain antibody, anti-T3E antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of Jurkat cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) stained using anti-human CD3 activation epitope (APA1/1) FITC antibody (concentration in sample 5 μg/ml).

Flow cytometry intracellular staining pattern of Jurkat cell suspension using anti-human CD3 activation epitope (APA1/1) FITC antibody (concentration in sample 5 μg/ml).