CD3 antibody (FITC)

• Catalog Number: orb44594
• Category: Antibodies
• Description: Hamster monoclonal antibody against CD3 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: 145-2C11
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Armenian Hamster IgG kappa
• Immunogen: Mouse BM10-37 cytotoxic T lymphocytes
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-3 μg/ml.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD3
• Entrez: 12501
• UniProt ID: P22646
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody (FITC), anti Cd247 antibody (FI
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD3 positive cells (red-filled) from CD3 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) FITC antibody (concentration in sample 1 μg/ml).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) FITC antibody (concentration in sample 1 μg/ml).