CD28 antibody

• Catalog Number: orb43723
• Category: Antibodies
• Description: Mouse Monoclonal to CD28.
• Clonality: Monoclonal
• Clone Number: CD28.2
• Tested applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-12 µg/ml. Western blotting: Recommended dilution: 1-2 µg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD28
• Entrez: 940
• UniProt ID: P10747
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD28 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-12 µg/ml.Western blotting: Recommended dilution: 1-2 µg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.

Western blotting analysis of human CD28 using mouse monoclonal antibody CD28.2 on lysates of Jurkat cells and THP1 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD28 was detected around 35-40 kDa.