CD28 Antibody

• Catalog Number: orb1252803
• Category: Antibodies
• Description: CD28 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C28/77
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human CD28 protein was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD28
• UniProt ID: P10747
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: T-cell-specific surface glycoprotein CD28, TP44, C
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE Analysis of Purified, BSA-Free CD28 Antibody (clone C28/77). Confirmation of Integrity and Purity of the Antibody.