CD28 antibody

• Catalog Number: orb1152457
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD28
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 15E8
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original mouse antibody was generated by immunizing mice with human T lymphocytes.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD28
• UniProt ID: P10747
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: T-cell-specific surface glycoprotein CD28; TP44
• Note: For research use only
• Application notes: This antibody works independently of TcR/CD3 and activation of protein kinase C (PKC) for activation of T cells and nehances cytokine secretion (PMID: 1647959). This antibody is capable of stimulating T cells in combination with either murine OKT3 antibody or CDR-mutated OKT3 Fab variants. It was found that cross-linking of specific tumor antigens with the T-cell-associated CD3 and CD28 antigens can increase IL-2 secretion, proliferation and antigen-specific cytotoxicity in resting T cells. This cross-linking can be achieved effectively by bispecific monoclonal antibodies (BiMAb) with specificity for both the tumor antigen and CD3 or CD28 antigen. A combination of bispecific antibodies BiMAb OKT3/HRS-3 with reactivity against both CD3 and Hodgkin's-lymphoma-associated CD30 antigen and BiMAb 15E8/HRS-3 with reactivity to both CD28 and CD30 antigen was able to activate resting T cells, represented by Jurkat cells (CD3+/CD28+) to produce IL-2 (PMID: 7686889). This antibody was also used in another study wherein the combined use of two CD3 x CD19 plus CD28 x CD22 bispecific antibodies induced optimal interleukin 2 secretion by Jurkat T-cell acute lymphocytic leukemia cells in the presence of target B-cells (PMID: 7689932). This antibody also provides a co-stimulatory signal to T cells. The scFv fragment of this antibody induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1×anti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype (PMID: 10913626). The humanzied version of this antibody is available on request.
• Expiration Date: 12 months from date of receipt.