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Catalog Number | orb43725 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD28. |
Clonality | Monoclonal |
Clone Number | CD28.2 |
Tested applications | FC |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 kappa |
Immunogen | DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD28 |
Entrez | 940 |
UniProt ID | P10747 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD28 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD28 (CD28.2) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
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