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Catalog Number | orb198183 |
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Category | Antibodies |
Description | Mouse polyclonal antibody to CD8, CD28 (FITC, PE) |
Species/Host | Mouse |
Tested applications | FACS |
Reactivity | Human |
Isotype | IgG1 (F)/IgG1 (PE) |
Immunogen | CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line. |
Concentration | Titered for flow cytometry |
Form/Appearance | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
Conjugation | FITC/RPE |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. |
UniProt ID | P01732, P10747 |
Storage | Product should be stored at 4-8°C. DO NOT FREEZE |
Buffer/Preservatives | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
Alternative names | CD8 FITC - CD28 PE Read more... |
Note | For research use only |
Application notes | PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20º C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC and PE. Antibodies should be stored at 4-8oC. Mabs should not be frozen. Reagents are stable for the period shown on the vial label when stored properly. |
Expiration Date | 12 months from date of receipt. |
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