Cd276 Antibody

• Catalog Number: orb1671831
• Category: Antibodies
• Description: Cd276 Antibody
• Clonality: Recombinant
• Clone Number: MJ18
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1–242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Cd276
• UniProt ID: Q8VE98
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD276, B7H3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-B7-H3 antibody MJ18 (orb1671831) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using orb1671831. For detection- a 1:4000 dilution of HRP-loat anti-ribody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of MJ18 (orb1671831) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671831- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.