CD276 Antibody

• Catalog Number: orb1239243
• Category: Antibodies
• Description: CD276 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2A7
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: B7-H3 antibody was raised against the extracellular domain of human B7-H3
• Concentration: 1 mg/mL
• Dilution range: B7-H3 antibody can be used for detection of B7-H3 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 57 kDa Observed: 100 kDa
• Target: CD276
• UniProt ID: Q5ZPR3
• NCBI: NP_001019907
• Storage: B7-H3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-
• Note: For research use only
• Application notes: B7-H3 antibody can be used for detection of B7-H3 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.

Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml. Green: B7-H3 Antibody [2A7] (orb1239243) Blue: DAPI staining

Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml. Green: B7-H3 Antibody [2A7] (orb1239243) Blue: DAPI staining

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.

Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.

Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with orb1240155, orb1239243, orb1239244, orb1239247, and orb1239231 antibodies at decreasing concentrations.