CD274 antibody

• Catalog Number: orb18977
• Category: Antibodies
• Description: Goat polyclonal antibody to CD274
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 33.3; 20.2
• Target: CD274 / PD-L1
• Entrez: 29126
• Protein Sequence: CKKQSDTHLEET
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Anti-CD274 antibody, anti-Programmed cell death 1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Skel;etal Muscle shows staining of the striated muscular fibres. Recommended concentration, 4-6ug/ml. Data obtained with a previous batch.WB: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). Recommended concentration: 0.3-1.0 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Heart lysate using CD274 antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using CD274 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using CD274 antibody

Immunofluorescence analysis of paraformaldehyde-fixed AA431 cells using CD274 antibody

Western blot analysis of U2OS cell lysate using CD274 antibody