CD274 Antibody

• Catalog Number: orb1671421
• Category: Antibodies
• Description: CD274 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human PD-L1 Fc. Anti-Human PD-L1 Fc-specific antibody was purified by affinity chromatography employing an immobilized Human PD-L1 Fc matrix.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CD274
• UniProt ID: Q9NZQ7
• NCBI: Q9NZQ7
• Storage: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Programmed Death Ligand 1, Programmed cell death 1
• Note: For research use only
• Application notes: Sandwich ELISA: To detect Human PD-L1 Fc by sandwich ELISA (using 100μl/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biorbyt’s Biotinylated Anti-Human PD-L1 Fc as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PD-L1 Fc. Western Blot To detect Human PD-L1 Fc by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human PD-L1 Fc by sandwich ELISA (using 100 μl/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PD-L1 Fc as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PD-L1 Fc.

To detect Human PD-L1 Fc by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.