CD274 Antibody

• Catalog Number: orb1239818
• Category: Antibodies
• Description: CD274 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1D7
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: PD-L1 antibody was raised against the extracellular domain of human PD-L1.
• Concentration: 1 mg/mL
• Dilution range: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 45 kDa
• Target: CD274
• UniProt ID: Q9NZQ7
• NCBI: NP_054862
• Storage: PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: PD-L1 Antibody: Programmed cell death 1 ligand-1,
• Note: For research use only
• Application notes: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 μg/ml

Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 μg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining

Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 μg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining

Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 μg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 μg/mL.

Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 μg/mL.

A sandwich ELISA was performed using the anti-PD-L1 mAb orb1239818 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-L1 mAb orb1238288 (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 20 pg.