CD273 antibody (PE)

• Catalog Number: orb414547
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD273 conjugated to PE
• Clonality: Monoclonal
• Clone Number: 24F.10C12
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: human CD273 cDNA
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD273
• Entrez: 80380
• UniProt ID: Q9BQ51
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-PDCD1LG2 antibody, anti-B7DC antibody, anti-B
• Note: For research use only
• Application notes: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension) and anti-human CD11c (BU15) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD273 positive CD11c positive cells (red-filled) from CD273 negative CD11c negative cells (black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).

Flow cytometry surface staining pattern of human GM-CSF and Il-4 stimulated peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) PE antibody (10 µl reagent per milion cells in 100 µl of cell suspension).