CD273 antibody

• Catalog Number: orb1473692
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD273
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD273. The exact sequence is proprietary.
• Dilution range: WB (1/1000 - 1/4000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PDCD1LG2
• Entrez: 80380
• UniProt ID: Q9BQ51
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: B7DC; CD273; PDCD1L2; PDL2; Programmed cell death
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD273 expression in Nontransfected 293T (A), Transfected PDL2transfected 293T (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 40 kD)

Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.