CD272 Antibody

• Catalog Number: orb1088502
• Category: Antibodies
• Description: CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.
• Clonality: Monoclonal
• Clone Number: MIH26
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG2a kappa
• Immunogen: cells transfected with human CD272
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD272
• Entrez: 151888
• UniProt ID: Q7Z6A9
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-BTLA1 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD272 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD272 (MIH26) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD272 (MIH26) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).