CD26 Rabbit Polyclonal Antibody

SKU: orb13310

Description

CD26 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC
• Dilution Range: ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD26 (661-766/766aa)
• Target: DPP4
• Molecular Weight: 110 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADABP; ADCP2; CD26; DPPIV; TP103; Dpp-4; THAM; DPP4_HUMAN; DPP4; Adenosine deaminase complexing protein 2 (ADCP-2); Dipeptidyl peptidase IV (DPP IV); T-cell activation antigen CD26; 3.4.14.5; DPP4_MOUSE; Thymocyte-activating molecule (THAM); DPP4_RAT; Bile canaliculus domain-specific membrane glycoprotein; GP110 glycoprotein;

Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD26 antibody (orb13310), Dilution: 3 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (Human melanoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (orb13310) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (orb13310) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

DPP4

UniProt

P27487