CD24 Rabbit Polyclonal Antibody

SKU: orb2941

Description

CD24 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD24 (27-53/76aa)
• Target: CD24
• Molecular Weight: 30-45 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD 24; CD24 antigen(small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; Cd24a; Ly-52; CD24_HUMAN.

Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-CD24 antibody (orb2941), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Bone tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Mouse Cerebrum tissue lysates, Primary: Anti-CD24 (orb2941) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 8 kD, Observed band size: 33 kD.

Quick Database Links

Gene Symbol

CD24

UniProt

P24807