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Catalog Number | orb316554 |
---|---|
Category | Antibodies |
Description | CD22 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 95348 MW |
UniProt ID | P20273 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | B-cell receptor CD22;B-lymphocyte cell adhesion mo Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of Raji cells using anti-CD22 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CD22 using anti-CD22 antibody.Lane 1:human raji cell;2:rat thymus tissue;3:mouse thymus tissue.
IHC analysis of CD22 using anti-CD22 antibody. CD22 was detected in a paraffin-embedded section of human tonsil tissue.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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