CD200R Rabbit Polyclonal Antibody

• Catalog Number: orb159257
• Category: Antibodies
• Description: CD200R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human CD200R (151-250/325aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CD200R1
• UniProt ID: Q8TD46
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD 200R; CD200 cell surface glycoprotein receptor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hepg2 Cells (blue). Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody, dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated (orb159257) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: hela (Human) Cell Lysate at 30 ug, Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD200R (orb159257) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 58 kD.