CD200R Rabbit Polyclonal Antibody

• Catalog Number: orb156302
• Category: Antibodies
• Description: CD200R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human CD200R (201-300/325aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: CD200R1
• UniProt ID: Q8TD46
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD 200R; CD200 cell surface glycoprotein receptor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Mycobacterium bovis protein (Cat. orb1516547) at 2 µg/mL (100 µl/well) can bind Rabbit Anti-CD200R Antibody, the EC50 is 158 ng/ml.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-CD200R (orb156302) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD200R (orb156302) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD200R Polyclonal Antibody, Unconjugated (orb156302) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.