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    CD2 antibody (PE)

    CD2 antibody (PE)

    Catalog Number: orb1293814

    DispatchUsually dispatched within 5-10 working days
    $ 359.00
    Catalog Numberorb1293814
    CategoryAntibodies
    DescriptionMouse monoclonal antibody against CD2 conjugated to PE
    Species/HostMouse
    ClonalityMonoclonal
    Clone NumberRPA-2.10
    Tested applicationsFC, IF
    ReactivityHuman
    ImmunogenNative purified human CD2.
    Dilution range10 μl / assay
    Form/AppearanceMouse IgG1. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
    ConjugationPE
    TargetCD2
    Entrez914
    UniProt IDP06729
    SourceMouse
    StorageShipped and store at 4°C for one year. Do not freeze.
    Buffer/PreservativesMouse IgG1. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
    Alternative namesSRBC; T-cell surface antigen CD2; Erythrocyte rece
    Read more...
    NoteFor research use only
    Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    Expiration Date12 months from date of receipt.
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