CD166 Rabbit Polyclonal Antibody

• Catalog Number: orb10298
• Category: Antibodies
• Description: CD166 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD166 (451-583/583aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 62 kDa
• Target: ALCAM
• UniProt ID: Q13740
• RRID: AB_10753266
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: activated leukocyte cell adhesion molecule; ALCAM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Molt-4 Cells (blue). Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (1 µg/1x10^6 cells) for 30 min on ice. Acquisition of 20000 events was performed.

Sample: HepG2 Cell (Human) Lysate at 30 ug, Primary: Anti-CD166 (orb10298) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62/70 kD.

Sample: MCF-7 Cell (Human) Lysate at 30 ug, Primary: Anti-CD166 (orb10298) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62/70 kD.