CD154 antibody (FITC)

• Catalog Number: orb44603
• Category: Antibodies
• Description: Hamster Monoclonal to CD154.
• Clonality: Monoclonal
• Clone Number: MR-1
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Armenian Hamster IgG1
• Immunogen: Activated mouse Th1 clone D1.6
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-3 μg/ml; positive control: 6-8 hour activated murine splenocytes.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD154
• Entrez: 959
• UniProt ID: P29965
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Hamster Monoclonal to CD154 (mouse) antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-3 μg/ml; positive control: 6-8 hour activated murine splenocytes.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of murine stimulated (PMA + Ionomycin) lymphocytes using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml) and anti-mouse CD3 (145-2C11) APC antibody (concentration in sample 2 μg/ml).

Separation of murine CD154 positive CD3 positive lymphocytes (red-filled) from CD154 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine stimulated (PMA + Ionomycin) splenocyte suspension stained using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml).

Flow cytometry surface staining pattern of murine stimulated (PMA + Ionomycin) splenocyte suspension stained using anti-mouse CD154 (MR-1) FITC antibody (concentration in sample 1 μg/ml).