CD154 antibody (FITC)

• Catalog Number: orb421975
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD154 conjugated to FITC
• Clonality: Monoclonal
• Clone Number: 24-31
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human CD154 fusion protein
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD154
• Entrez: 21947
• UniProt ID: P27548
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD40L antibody, anti-CD40 ligand antibody, an
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry detection of CD154 / CD40L in IgE-activated human peripheral blood with anti-CD154 / CD40L (24-31) FITC. Orange = blank, blue = CD154 negative cells, red = CD154 positive cells.

Separation of PMA/Ionomycine activated PBMCs stained using anti-human CD154 (24-31) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from PMA/Ionomycine activated PBMCs stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 3 μg/ml, same as CD154 FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of PMA/Ionomycine activated PBMCs.