CD15 Antibody / SSEA-1

• Catalog Number: orb385947
• Category: Antibodies
• Description: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on > 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Purified human neutrophils were used as the immunogen for the CD15 antibody.
• Dilution range: Flow cytometry: 2-4ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 5-10ug/ml for 30 min at RT
• Purity: Protein A affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD15 antibody is available for research use only.
• UniProt ID: P22083
• Storage: Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD15 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human Hodgkin's lymphoma using CD15 antibody