CD14 Antibody

• Catalog Number: orb334580
• Category: Antibodies
• Description: CD14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD14 recombinant protein (Position: N65-D327). Human CD14 shares 69.8% and 66.8% amino acid (aa) sequence identity with mouse and rat CD14, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 40076 MW
• UniProt ID: P08571
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Monocyte differentiation antigen CD14 ;Myeloid cel
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CD14 using anti-CD14 antibody.Lane 1:human THP-1 cell;2:human Raji cell.

IHC analysis of CD14 using anti-CD14 antibody. CD14 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of CD14 using anti-CD14 antibody. CD14 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of CD14 using anti-CD14 antibody. CD14 was detected in a paraffin-embedded section of human liver cancer tissue.