CD14 antibody

• Catalog Number: orb1429922
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD14.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14105
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:25, WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P-Leica: 1:1000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 40076
• Target: CD14
• UniProt ID: P08571
• NCBI: NP_000582.1, NP_001167575.1, NP_001167576.1, NP_001035110.1
• Alternative names: Monocyte differentiation antigen CD14, Myeloid cel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of A549 cells labeling CD14 with orb1429922 at 1:25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG(green).The nuclear counter stain is DAPI(blue).

Western blot:All lanes:Anti-CD14 Antibody (N-term) at 1:2000 dilution.Lane 1:Human lung tissue lysate;Lane 2:Human liver tissue lysate;Lane 3:A549.

Western blot:All lanes:Anti-CD14 Antibody (N-term) at 1:1000 dilution.Lane 1:Human lung tissue lysate;Lane 2:Human liver tissue lysate.

Western blot:All lanes:Anti-CD14 Antibody (N-term) at 1:1000 dilution.Lane 1:THP-1;Lane 2:Human lung tissue lysate;Lane 3:Human liver tissue lysate;Lane 4:A549.

Western blot:All lanes:Anti-CD14 Antibody (N-term) at 1:2000 dilution.Lane 1:Human liver lysate;Lane 2:Human lung lysate.

Immunohistochemical analysis of human liver tissue using orb1429922 performed on the Leica® BOND RXm at 1:1000 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow cytometric analysis of Jurkat cells stained with orb1429922(green line) at 1:25 dilution.Secondary antibody was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed.Isotype control antibody (blue line) was rabbit IgG1.