CD133 antibody

• Catalog Number: orb766797
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD133
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the N-terminal region of human PROM1. AA range:41-90
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 100
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PROM1 antibody, anti-Prominin-1 antibody, ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western Blot analysis of A549, SW620, HT29, Jurkat, mouse kidney, mouse lung cells using CD133 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).