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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | IL5RA |
| Tag | His Tag |
| MW | 37.7 kDa |
| Purity | >85% as determined by SDS-PAGE. |
| Protein Sequence | Asp 21 - Glu 335 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37.6 KDa. Observed: 48 KDa, reducing conditions
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.7 KDa. Observed: 45-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-5RA Protein [orb2994171]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.5 KDa. Observed: 70-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL5RA Protein, hFc Tag [orb757475]
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The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgHuman IL5RA Protein, His Tag [orb689433]
Unconjugated
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg

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The purity of rh IL5RA/CD125 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
CD125 Recombinant Protein (orb1227988)
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