CD11c Rabbit Polyclonal Antibody (FITC)

SKU: orb14009

Description

CD11c Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha X (501-600/1163aa)
• Target: ITGAX
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin alpha-X; integrin alpha-X precursor; ITGAX; SLEB6; CD11C(p150) alpha polypeptide; complement receptor 4; integrin aX; CD11 antigen-like family member C; leukocyte adhesion receptor p150, 95; leukocyte adhesion glycoprotein p150, 95 alpha chain; ITAX_HUMAN.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD11c antibody (orb14009), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD11c antibody (orb14009), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

ITGAX

References

Zhang, Hong et al. Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model PLoS One, 12, e0188960 (2017)

PubMed: 29206859