CD11c Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb14009
• Category: Antibodies
• Description: CD11c Rabbit Polyclonal Antibody (FITC)
• Target: ITGAX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha X (501-600/1163aa)
• RRID: AB_10736705
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Integrin alpha-X; integrin alpha-X precursor; ITGA
• Note: For research use only

References

Zhang, Hong et al. Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model PLoS One, 12, e0188960 (2017)

PubMed: 29206859

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD11c antibody (orb14009), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD11c antibody (orb14009), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.