CD11a antibody

• Catalog Number: orb44402
• Category: Antibodies
• Description: Mouse Monoclonal to CD11a.
• Clonality: Monoclonal
• Clone Number: MEM-83
• Tested applications: FA, FC, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human peripheral blood lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow cytometry: Recommended dilution: 1 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD11a
• Entrez: 3683
• UniProt ID: P20701
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-CD11a antibody, anti-LFA-1 alpha chain antibo
• Note: For research use only
• Application notes: Functional application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow cytometry: Recommended dilution: 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-83) purified antibody (azide free, concentration in sample 1 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (azide free, concentration in sample 1 μg/ml) GAM APC.