CD117 Antibody

• Catalog Number: orb1252741
• Category: Antibodies
• Description: CD117 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C117/370
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for this CD117 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD117
• UniProt ID: P10721
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Mast/stem cell growth factor receptor Kit, SCFR, P
• Note: For research use only
• Application notes: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

Formalin-paraffin human gastrointestinal stromal tumor (GIST) stained with CD117 antibody (C117/370).