c-Kit antibody

• Catalog Number: orb137105
• Category: Antibodies
• Description: Goat polyclonal antibody to c-Kit
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Porcine
• Concentration:

  1-3 μg/ml

• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 110; 109
• Target: KIT / CD117
• Entrez: 3815
• Protein Sequence: KRDSFICSKQEDH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-C Kit antibody, anti-c-kit antibody, anti-CD
• Note: For research use only
• Application notes: Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.
• Expiration Date: 12 months from date of receipt.

WB analysis of Human Hippocampus lysate (Lane 1) + peptide (Lane 2) using c-Kit antibody

Immunofluorescence analysis of HEK293 cells using c-Kit antibody

Immunofluorescence analysis of HeLa cells using c-Kit antibody

Flow cytometric analysis of MCF7 cells using c-Kit antibody

Immunohistochemical staining of Human Prostate using c- kit antibody