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Catalog Number | orb1252081 |
---|---|
Category | Antibodies |
Description | CD117 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CLDA117 |
Tested applications | IF, IHC |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for this CD117 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunofluorescence: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CD117 |
UniProt ID | P10721 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Mast/stem cell growth factor receptor Kit, SCFR, P Read more... |
Note | For research use only |
Application notes | Immunofluorescence: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human gastrointestinal stromal tumor (GIST) and CD117 antibody (CLDA117).
ELISA, FC, IF, IHC, WB | |
Bovine, Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |
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