CCT5 Rabbit Polyclonal Antibody

SKU: orb582501

Description

Rabbit polyclonal antibody to CCT5

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Yeast, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CCT5
• Target: CCT5
• Protein Sequence: Synthetic peptide located within the following region: NDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAE
• Molecular Weight: 60kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCTE, HEL-S-69, PNAS-102, CCT-epsilon, TCP-1-epsilon

Lanes: 1. Mouse WT brain extract (80 ug) 2. Rat brain extract (80 ug), Primary Antibody dilution: 2 ug/ml, Secondary Antibody: IRDye 800CW goat anti-rabbit, Secondary Antibody dilution: 1:20000, Gene Name: CCT5, CCT5 is supported by BioGPS gene expression data to be expressed in HEK293.

Rabbit Anti-CCT5 Antibody, Catalog Number: orb582501, Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue, Observed Staining: Cytoplasmic, Primary Antibody Concentration: 1:100, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

Application: Western blotting, Species+tissue/cell type: Mouse WT brain and Rat brain. How many ug's of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80 ug) 2. Rat brain extract (80 ug), Primary antibody dilution: 2 ug/ml, Secondary antibody: IRDye 800CW goat anti-rabbit, Secondary antibody dilution: 1:20000.

WB Suggested Anti-CCT5 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:12500, Positive Control: Human brain.

Quick Database Links

Gene Symbol

CCT5

UniProt

P48643

RefSeq (Protein):NP_036205