CCT3 antibody

• Catalog Number: orb377971
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CCT3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human CCT3
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CCT3
• Entrez: 295230, 12462, 7203
• UniProt ID: P49368, Q6P502, P80318
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: CCTG; TRIC5; T-complex protein 1 subunit gamma; TC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CCT3 expression in MCF7 (A), THP1 (B), mouse ovary (C), mouse thymus (D), rat spleen (E), rat heart (F) whole cell lysates. (Predicted band size: 56; 60 kD; Observed band size: 60 kD)

Immunofluorescent analysis of CCT3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.