CCT3 Antibody

• Catalog Number: orb1264526
• Category: Antibodies
• Description: CCT3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Mouse, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the Central region of human CCT3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CCT3
• UniProt ID: P49368
• NCBI: P49368
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: T-complex protein 1 subunit gamma, TCP-1-gamma, CC
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CCT3 Antibody in MCF7, CEM, K562, HL-60, Hela cell line lysates (35 ug/lane)

Western blot analysis of CCT3 using rabbit polyclonal CCT3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2).

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.