CCR5 Rabbit Polyclonal Antibody

• Catalog Number: orb13298
• Category: Antibodies
• Description: CCR5 Rabbit Polyclonal Antibody
• Target: CCR5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CCR-5 (201-300/352aa)
• UniProt ID: P51681
• RRID: AB_10749533
• MW: 40 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C-C chemokine receptor 5; CCR5; CC-CKR-5; CCR-5; C
• Research Area: Alzheimer's Disease, CC Chemokines, Chemokines, G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CCR5 antibody (orb13298), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Human THP-1 cell lysates, Lane 2: Human U937 cell lysates, Primary: Anti-CCR-5 (orb13298) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 43 kD.