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CCL3L1 Antibody
Catalog Number: orb344656
| Catalog Number | orb344656 |
|---|---|
| Category | Antibodies |
| Description | CCL3L1 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Bovine, Porcine |
| Isotype | IgG |
| Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1 protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | WB: 1 µg/ml |
| Form/Appearance | Lyophilized |
| Purity | This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine CCL3L1 protein. A BLAST analysis was used to suggest cross-reactivity with CCL3L1 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against CCL3L1 from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | A1YM34 |
| NCBI | 57527998 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-CCL3L1 Antibody, CCL3L3, Chemokine (C- Read more... |
| Note | For research use only |
| Application notes | This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract. |
| Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's protein-A purified anti-swine CCL3L1 antibody shows detection of recombinant swine CCL3L1 at 7.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with Biorbyt's Anti-swine CCL3L1. After washing, membrane was probed with Dylight ™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
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