CCL3 Antibody

• Catalog Number: orb1272676
• Category: Antibodies
• Description: CCL3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1a (human MIP-1 alpha). Human MIP-1a specific antibody was purified by affinity chromatography employing immobilized hMIP-1a matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hMIP-1a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.SandwichTo detect hMIP-1a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human MIP-1a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a. Western Blot:To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL3
• UniProt ID: P10147
• NCBI: P10147
• Storage: MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hMIP-1a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a.SandwichTo detect hMIP-1a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human MIP-1a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a. Western Blot:To detect hMIP-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
• Expiration Date: 12 months from date of receipt.

To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1-alpha (orb1272675) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-1-alpha.

To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human MIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.