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CCL25 Rabbit Polyclonal Antibody
Catalog Number: orb4792
| Catalog Number | orb4792 |
|---|---|
| Category | Antibodies |
| Description | CCL25 Rabbit Polyclonal Antibody |
| Target | CCL25 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human CCL25 (21-120/150aa) |
| UniProt ID | O15444 |
| RRID | AB_10941804 |
| MW | 14 kDa |
| Tested applications | IF, IHC-Fr, IHC-P |
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CCL25_HUMAN; C-C motif chemokine 25; Chemokine TEC Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (orb4792) at 1:400 overnight at 4°C, followed by a conjugated secondary (orb868589) at 1:500 for 90 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (orb4792) at 1:400 overnight at 4°C, followed by a conjugated secondary (orb868589) at 1:500 for 90 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (orb4792) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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