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Catalog Number | orb1272361 |
---|---|
Category | Antibodies |
Description | CCL20 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3β (human Macrophage Inflammatory Protein-3 beta). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3β (100 ng/mL), a concentration of 2.7 4.0 μg/mL of this antibody is required. ELISA:To detect hMIP-3β by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3β. Sandwich:To detect hMIP-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-3βas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3β. Western Blot:To detect hMIP-3β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CCL20 |
UniProt ID | P78556 |
NCBI | P78556 |
Storage | MIP-3B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, MIP-3-alp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect Human MIP-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MIP-3-beta (orb1272360) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-beta.
To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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