CCL2 Antibody

• Catalog Number: orb1272404
• Category: Antibodies
• Description: CCL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP 1/MCAF (human Macrophage/Monocyte chemotactic protein-1).
• Concentration: batch dependent
• Dilution range: ELISA:To detect hMCP-1/MCAF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-1/MCAF.Sandwich:To detect hMCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-1(MCAF)as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1(MCAF). Western Blot:To detect hMCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CCL2
• UniProt ID: P13500
• NCBI: P13500
• Storage: MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect hMCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human MCP-1 (MCAF) (orb1272403) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1 (MCAF).

To detect hMCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hMCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 (MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml and a two-hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.