CCDC11 Polyclonal Antibody

SKU: orb1416895

Description

Rabbit polyclonal antibody to CCDC11.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Target: CFAP53 (HGNC:26530)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cilia- and flagella-associated protein 53 {ECO:0000312|HGNC:HGNC:26530}, Coiled-coil domain-containing protein 11 {ECO:0000312|HGNC:HGNC:26530}, CFAP53 (HGNC:26530)

Sample: Testis (Mouse) Lysate at 40 ug. Primary: Anti- CCDC11 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 57 kD. Observed band size: 63 kD.

Sample: A431 (Human) Cell Lysate at 30 ug. Primary: Anti- CCDC11 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 57 kD. Observed band size: 63 kD.

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC11) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Quick Database Links

Gene Symbol

CFAP53 (HGNC:26530)

UniProt

Q96M91