CBX5 antibody

• Catalog Number: orb19140
• Category: Antibodies
• Description: Goat polyclonal antibody to CBX5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Canine, Human, Mouse
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 22.2
• Target: CBX5 / HP1-Alpha
• Entrez: 23468
• Protein Sequence: NKRKSNFSNSADDIK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CBX5 antibody, anti HP1-ALPHA antibody, anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Uterus shows strong nuclear staining in the glandular epithelial cells. Recommended concentration, 2-3ug/ml.WB: Approx. 22kDa band observed in lysates of Human fibroblast cell lines A431 and HeLa (calculated MW of 22.2kDa according to NP_036249.1). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of A43N (A) and K562 (B) cell lysate using CBX5 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using CBX5 antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using CBX5 antibody.