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Catalog Number | orb1671547 |
---|---|
Category | Antibodies |
Description | CBX5 Antibody |
Clonality | Recombinant |
Clone Number | RAB-C133 |
Tested applications | ChIP, ELISA |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX5 protein under non-denaturing conditions. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | CBX5 |
UniProt ID | P45973 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | p25, Chromobox protein homolog 5, Heterochromatin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot using anti-CBX5 antibody R Nuclear lysates of HeLa(A) - HEK293(B) - A431(C) - MCF7(D) - Jurkat(E) and K562(F) cells (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion at 0.003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the Anti-CBX5 antibody R Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-CBX5. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671547- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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