CBX5 Antibody

• Catalog Number: orb1247696
• Category: Antibodies
• Description: CBX5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-NKRKSNFSNSADDIK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CBX5
• UniProt ID: P45973
• NCBI: NP_001120794.1, NP_001120793.1, NP_036249.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha ho
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247696 (0.1 ug/ml) staining of K562 (A), MCF7 (B) and NIH3T3 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247696 (2 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247696 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).