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CBX5 Antibody

Catalog Number: orb1247696

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1247696
CategoryAntibodies
DescriptionCBX5 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IHC, WB
Predicted ReactivityCanine
ReactivityHuman, Mouse
ImmunogenThe immunogen for this antibody is: C-NKRKSNFSNSADDIK
Concentration500 ug/mL
Dilution rangePeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Form/AppearanceLiquid
ConjugationUnconjugated
TargetCBX5
UniProt IDP45973
NCBINP_001120794.1, NP_036249.1, NP_001120793.1
StorageAliquot and store at -20˚°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesCBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha ho
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Expiration Date12 months from date of receipt.
CBX5 Antibody

orb1247696 (0.1 ug/ml) staining of K562 (A), MCF7 (B) and NIH3T3 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

CBX5 Antibody

orb1247696 (2 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

CBX5 Antibody

orb1247696 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).

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