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CBX5 Antibody

SKU: orb1247696

Description

CBX5 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, FC, IHC, WB
ReactivityHuman, Mouse
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4µg/mL.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NKRKSNFSNSADDIK
TargetCBX5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha , chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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